منابع مشابه
Plaque assay for Rickettsia rickettsii.
A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick or green monkey kidney monolayer cell cultures with either an agarose or special Noble agar overlay. Plaques were counted in 6 days and resultant titers correlated well with ld(50) end points obtained by a standard assay in embryonated eggs. Identification of the plaque-forming organisms was...
متن کاملIntranuclear growth of Rickettsia rickettsii.
The ability of the spotted fever agent, Rickettsia rickettsii, to grow not only in the cytoplasm but also in the nucleus of infected cells was first noted by Wolbach (12) in epithelial cells of the gut, hypoderm and salivary gland of infected ticks; it has subsequently been established for all spotted fever group rickettsiae investigated, especially when they were maintained on mammalian tissue...
متن کاملGenomic comparison of virulent Rickettsia rickettsii Sheila Smith and avirulent Rickettsia rickettsii Iowa.
Rickettsia rickettsii is an obligate intracellular pathogen that is the causative agent of Rocky Mountain spotted fever. To identify genes involved in the virulence of R. rickettsii, the genome of an avirulent strain, R. rickettsii Iowa, was sequenced and compared to the genome of the virulent strain R. rickettsii Sheila Smith. R. rickettsii Iowa is avirulent in a guinea pig model of infection ...
متن کاملAssessment of real-time PCR assay for detection of Rickettsia spp. and Rickettsia rickettsii in banked clinical samples.
Two novel real-time PCR assays were developed for the detection of Rickettsia spp. One assay detects all tested Rickettsia spp.; the other is specific for Rickettsia rickettsii. Evaluation using DNA from human blood and tissue samples showed both assays to be more sensitive than nested PCR assays currently in use at the CDC.
متن کاملComplementation of Rickettsia rickettsii RelA/SpoT restores a nonlytic plaque phenotype.
Spotted fever group rickettsiae are known to produce distinct plaque phenotypes. Strains that cause lytic infections in cell culture form clear plaques, while nonlytic strains form opaque plaques in which the cells remain intact. Clear plaques have historically been associated with more-virulent species or strains of spotted fever group rickettsiae. We have selected spontaneous mutant pairs fro...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1969
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.98.2.398-402.1969